Intact Genomics igNext™ NGS DNA Library Preparation Kit provides a reliable and affordable option for your NGS needs

●  Reliable
Utilizes the classic protocol for DNA library preparation on the Illumina platform, enabling steady and proven performance for various sample types.
●  Simple
End repair, dA-tailing and Adaptor ligation finished in the same tube, reducing clean up steps to minimize sample loss, and shortening process time to increase throughput.
●  Low Input
Designed for low input DNA amount (1 – 100 ng).
●  Flexible
Unique multiplex index primer sets provide a large number of indices (barcodes).
–Up to 48 x 48 , or 2,304 unique dual-index combinations – enables a multitude of options for library multiplexing
●  Good performance in large-scale multiplexing
Superb coverage, without individual NGS library QC.
–98% of ~1,500 individual samples (averaging up to 100kb for each sample) can have greater than 100X depth of coverage by a Hiseq Xten lane (2×150 bp)
–94% of ~1,500 individual samples (averaging up to 100kb for each sample) can have greater than 5X depth of coverage by a Miseq run (2×75 bp).
●   Affordable
Competitive prices for all reagents needed to prep NGS library (sample purification beads and unique indexes included!)

igNext™ NGS DNA Library Prep Kit for Illumina

igNext™ NGS DNA Library Prep Kit includes high-quality buffers and enzymes needed to make DNA fragment libraries for whole genome sequencing on the Illumina platform. This kit facilitates consistent and proven performance for various types of samples. For optimal performance, the igNext™ NGS DNA Library Prep Kit should be used in conjunction with our igNext™ unique, specific index primer sets.  These primer sets provide 48 unique dual indexes and up to 2,304 dual-index combinations, offering a huge range of options for library multiplexing.

Workflow

igNext NGS Library Prep Kit Workflow


igNext™ Dual Index Oligos

igNext™ Dual Index Oligos provide flexible choices to satisfy the need of customers in need of various scales of multiplexing. Based on the number of samples, we offer multiple igNext Index products.  igNext™ Dual Index Oligos include up to 48 unique i7 index primers and 48 unique i5 index primers. Indexing options include 1) combinatorial dual indices and 2) unique dual indices. Currently, our primers enable multiplexing of up to 2304 dual indexes and 48 unique dual indexes.

Options include:

  • 1 to 12 samples: igNext™ 12 Unique Dual Index Strip.
  • 13 to 48 samples: igNext™ 48 Unique Dual Index Plate Set A or Set B
  • 49 to 96 samples: igNext™ 48 Unique Dual Index Plate Set A and Set B
  • > 96 samples (and up to 2304 samples): Contact us for preparation of different sets of the 24 igNext™ 96 Combinatorial Dual Index Plates.