Intact Genomics (ig®) DirectPlate™ DH5-Alpha chemically competent E. coli cells a perfect choice for researchers looking to simplify their transformation workflow by eliminating time-consuming heat shock, lengthy incubations, and outgrowth procedures. Simply mix and directly plate! Intact Genomics ‘DirectPlate™’ technology allows researchers to simply mix DNA and cells a few seconds, then directly spread the mixture to an appropriate selection plate. Intact Genomics (ig®) DirectPlate™ DH5-Alpha chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Competent cell type: Chemically competent
Derivative of: DH5-Alpha
Species: E. coli
Transformation efficiency: ≥1 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
Shipping condition: Dry ice
Reagents Needed for One Reaction
DirectPlate™ DH5-Alpha competent cell: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
DirectPlate™ DH5-Alpha competent cells: -80 ºC
pUC19 control DNA: -20 ºC
Φ80 Δ(lacZ)M15 fhuA2 Δ(argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Follow these guidelines when using DirectPlate™ DH5-Alpha chemically competent E. coli.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times. 1013-13 1013-36