Description

Intact Genomics (ig®) DirectPlate™ DH5-Alpha chemically competent E. coli cells a perfect choice for researchers looking to simplify their transformation workflow by eliminating time-consuming heat shock, lengthy incubations, and outgrowth procedures. Simply mix and directly plate! Intact Genomics ‘DirectPlate™’ technology allows researchers to simply mix DNA and cells a few seconds, then directly spread the mixture to an appropriate selection plate. Intact Genomics (ig®) DirectPlate™ DH5-Alpha chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

DirectPlate 5-alpha chemically competent cells Protocol Comp

Specifications

Competent cell type:             Chemically competent
Derivative of:                         DH5-Alpha
Species:                                E. coli
Format:                                 Tubes
Transformation efficiency:      ≥1 x 108 cfu/µg pUC19 DNA
Blue/white screening:           Yes
Shipping condition:               Dry ice

Reagents Needed for One Reaction

DirectPlate™ DH5-Alpha competent cell:     50 µl
DNA (or pUC19 Control, 10 pg/µl):                  1 µl

Storage

DirectPlate™  DH5-Alpha competent cells:   -80 ºC
pUC19 control DNA:                                      -20 ºC

Genotype

Φ80 Δ(lacZ)M15 fhuA2 Δ(argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17

Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using DirectPlate™  DH5-Alpha chemically competent E. coli.

  • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
  • Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times. 1013-13 1013-36