Description

Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ HB101 chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ HB101 chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

DirectPlate 5-alpha chemically competent cells Protocol Comp

Specifications

Competent cell type:             Chemically competent
Species:                                E. coli
Format:                                 Tubes
Transformation efficiency:      ≥1 x 108 – 109 cfu/µg pUC19 DNA
Blue/white screening:            No
Shipping condition:                Dry ice

Reagents Needed for One Reaction

DirectPlate™ HB101 competent cell:                    50 µl
DNA (or pUC19 Control, 10 pg/µl):                          1 µl

Storage

DirectPlate™  HB101 competent cells:              -80 ºC
pUC19 control DNA:                                          -20 ºC

Genotype

F- Lambda- araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(rB-, mB-)

Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 – 109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using DirectPlate™ HB101 chemically competent E. coli.

  • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
  • Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times.

Comparison Testing

Intact Genomics has performed extensive comparison testing to prove our DirectPlate™ HB101 chemically competent cells have the highest transformation efficiency on the market.1017-12 1017-36