Description
Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ JM109(DE3) chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ JM109(DE3) chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Specifications
Competent cell type: Chemically competent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 108 – 109 cfu/µg pUC19 DNA
Blue/white screening: No
Shipping condition: Dry ice
Reagents Needed for One Reaction
DirectPlate™ JM109(DE3) competent cell: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
Storage
DirectPlate™ JM109(DE3) competent cells: -80 ºC
pUC19 control DNA: -20 ºC
Genotype
endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15]. IDE3
Quality Control
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 – 109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
General Guidelines
Follow these guidelines when using DirectPlate™ JM109(DE3) chemically competent E. coli.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times.
Comparison Testing
Intact Genomics has performed extensive comparison testing to prove our DirectPlate™ JM109(DE3) chemically competent cells have the highest transformation efficiency on the market.1021-12 1021-36