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E. coli DNA Polymerase I (Pol I)

$58.00$245.00


    500 Units (50 μl) - $58.00 (Cat.# 3266 )

    2,500 Units (250 μl) - $245.00 (Cat.# 3268 )

Category: .

Description

E.coli DNA Polymerase I is a DNA-dependent DNA polymerase which has both 3´→ 5´ and 5´→ 3´ exonuclease activities (1). The 5´→ 3´ exonuclease removes nucleotides from the growing DNA chain and allows nick-translation.

The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).

Eco1

Additional information

Units

500 Units (50 μl), 2,500 Units (250 μl)

Product Source
E. coli strain carrying recombinant E. coli polA gene.

Applications

  • Nick translation of DNA to generate highly specific DNA probes (2).
  • Second strand cDNA synthesis (3).

Product Includes

  1. DNA Polymerase I
  2. 10x DNA Polymerase I Reaction Buffer 

Storage Temperature
–20 °C      

Storage Buffer

50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM β-mercaptoethanol, 1 mM DTT and 50% (v/v) glycerol.

10X Reaction Buffer

500 mM Tris-HCl, 100 mM MgCl2, 50 mM dithiothreitol (DTT), pH 7.5 @ 25 °C.

Inactivation

Inactivated by heating at 75 °C for 20 min. 

Quality Control Assays

DNA Polymerase I is free from detectable endonuclease and RNase activities.

References

  1. Lehman, I. R (1981). In P.D. Boyer(Ed.), The Enzymes. 14A, 16-38. San Diego: Academic Press.
  2. Meinkoth, J and Wahl, G. M (1987). S. L. Berger and A. R. Kimmel (Ed.), Methods Enzymol.152, 91-94. San Diego: Academic Press.
  3. Gubler, U and Hoffmann, B. J (1983). Gene. 25, 263-269.

E. coli DNA Polymerase I (Pol I)

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