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Hot Start Taq 2x Master Mix

$40.00$210.00


    100 reactions (50 μl rxn vol) - $70.00 (Cat.# 3295 )

    500 reactions (50 μl rxn vol) - $210.00 (Cat.# 3296 )

    100 reactions (20 μl rxn vol) - $40.00 (Cat.# 3297 )

    500 reactions (20 μl rxn vol) - $130.00 (Cat.# 3299 )

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Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with the unique Intact Genomics 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Additional information

reaction

100 reactions (50 μl rxn vol), 500 reactions (50 μl rxn vol), 100 reactions (20 μl rxn vol), 500 reactions (20 μl rxn vol)

Applications

  • Routine PCR and RT-PCR
  • Primer extension
  • Colony PCR
  • Genotyping
  • Efficient for amplifying high GC content template DNA with Magic Enhancer.

 Product Includes

  1. Hot start Taq 2x master mix
  2. 5X Magic Enhancer

1x Master Mix Composition

10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl2, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Hot start Taq Polymerase.

Protocol

    1. Prepare a reaction mix according to the following table:

hot-start-taq-2x-master-mix-pic1

      1. Mix the reaction mixture thoroughly.
      2. Program the thermal cycler according to the manufacturer’s instructions.
      3. A typical PCR cycling program is outlined in the following table.

hot-start-taq-2x-master-mix-pic2

      1. Place the PCR tubes in the thermal cycler and start the cycling program.
      2. Analyze 5 μl of PCR products by agarose gel electrophoresis.

References:

    1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
    2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
    3. Longley, M.J., Bennett, S.E and Mosbaugh D.W. (1990) Nucleic Acids Res.18, 7317-7322.
    4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.

Hot Start Taq 2x Master Mix Manual

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