Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.
Competent cell type: Chemically competent
Derivative of: 5-alpha
Species: E. coli
Transformation efficiency: ≥1 x 109 cfu/µg pUC19
Blue/white screening: Yes
Shipping condition: Dry ice
Reagents Needed for One Reaction
ig™ 5-alpha chemically competent cells: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
Recovery medium: 1 ml
ig™ 5-alpha competent cells: -80 ºC
pUC19 control DNA: -20 ºC
Recovery medium: 4 ºC
Φ80 Δ(lacZ)M15 fhuA2 Δ(argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol. Transformation efficiency should be greater than 1×109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Follow these guidelines when using ig™ 5-alpha chemically competent cells.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.