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igNext™ NGS DNA Library Prep Kit for Illumina

$285.00$6,995.00

The igNext™ NGS DNA Library Prep Kit is a reliable, flexible, simple and affordable option for your NGS library preparation needs.


    igNext DNA Library Prep Kit with beads and index-12 Reactions - $435.00 (Cat.# 5116 )

    igNext DNA Library Prep Kit with beads and index(Set A)-48 Reactions - $1565.00 (Cat.# 5118 )

    igNext DNA Library Prep Kit with beads and index(Set A&B)-96 Reactions - $2395.00 (Cat.# 5119 )

    igNext DNA Library Prep Kit with beads-12 Reactions - $285.00 (Cat.# 5111 )

    igNext DNA Library Prep Kit with beads-96 Reactions - $1995.00 (Cat.# 5113 )

    igNext DNA Library Prep Kit with beads-384 Reactions - $6995.00 (Cat.# 5114 )

Description

The igNext™ NGS DNA Library Preperation Kit includes high-quality buffers and enzymes needed to make DNA fragment libraries for whole-genome sequencing on the Illumina platform. This kit facilitates consistent and proven performance for various types of samples. For optimal performance, the igNext™ NGS DNA Library Prep Kit should be used in conjunction with our unique, specific index primer sets.  These primer sets provide 48 unique dual index oligos and up to 2,304 dual-index combinations, offering a huge range of options for library multiplexing.  Link to the igNext™ Dual Index Oligos may be found in the ‘You may also like’ section at the bottom of this page.  Additional data and protocol information may be found in the tabs to the left.

Benefits of igNext™ NGS DNA Library Preparation Kit  

  • Reliable – Utilizes the classic protocol for DNA library preparation on the Illumina platform, enabling steady and proven performance for various sample types.
  • Simple – End repair, dA-tailing and Adaptor ligation finished in the same tube, reducing clean up steps to minimize sample loss, and shortening process time to increase throughput.
  • Low input – Designed for low input DNA amount (1 – 100 ng).
  • Flexible – Unique multiplex index primer sets provide a large number of indices (barcodes).
    • Up to 48 unique dual-index and 2,304 dual index combinations – enables a multitude of options for library multiplexing
  • Good performance in large-scale multiplexing – Superb coverage, without individual NGS library QC.
    • 98% of ~1,500 individual samples (averaging up to 100kb for each sample) can have greater than 100X depth of coverage by a Hiseq Xten lane (2×150 bp)
    • 94% of ~1,500 individual samples (averaging up to 100kb for each sample) can have greater than 5X depth of coverage by a Miseq run (2×75 bp).
  • Affordable – Competitive prices for all reagents needed to prep NGS library (sample purification beads and unique indexes included!)

Components

  • igNext™ End Prep Reaction Buffer (10X)
  • igNext™ End Prep Enzyme Mix
  • igNext™ Ligation Master Mix
  • igNext™ Universal Adaptor
  • igNext™ i7 HiFi PCR Master Mix (2X)
  • igNext™ NGS Library Prep with Sample Purification Beads (AMPure® XP Beads)
  • 1X TE Buffer

At Intact Genomics, we strictly test each batch of products ensuring they meet, or exceed, our published performance specifications.

Information on large volume requirements, customized packaging and specific index combination is available by contacting: sales@intactgenomics.com.

Additional information

NGS Product Type

igNext DNA Library Prep Kit with beads and index-12 Reactions, igNext DNA Library Prep Kit with beads and index(Set A)-48 Reactions, igNext DNA Library Prep Kit with beads and index(Set A&B)-96 Reactions, igNext DNA Library Prep Kit with beads-12 Reactions, igNext DNA Library Prep Kit with beads-96 Reactions, igNext DNA Library Prep Kit with beads-384 Reactions

The igNext™ NGS DNA Library Prep Kit provides a multitude of benefits for your NGS library preparation needs.  Further details on these benefits of this kit can be found in the link below:

The full manual and instructions for the igNext™ NGS DNA Library Preparation Kit may be found below.

Information on large volume requirements, customized packaging and specific index combination is available by contacting: sales@intactgenomics.com.

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