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igScript™ First Strand cDNA Synthesis Kit

$110.00$395.00


    25 reactions - $110.00 (Cat.# 4312 )

    100 reactions - $395.00 (Cat.# 4314 )

Description

igScript™ first strand cDNA synthesis kit includes 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer

[d(T)23VN] forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55 ºC.

Additional information

reaction

25 reactions, 100 reactions

Applications

  • First strand cDNA synthesis for PCR or RT-PCR
  • Gene expression data validation by using RT-PCR

 Benefits

  • Active at temperatures up to 55° C.
  • Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.

Product Includes

  1. 5x igScript™ master mix
  2. Oligo d(T)23 VN primer (50 µM)
  3. Random hexamer primer mix (60 µM)
  4. Nuclease free water

Storage Temperature

–20 °C

  1. In a sterile micro-centrifuge tube, add the following components on ice:
    igscript-first-strand-cdna-synthesis-kit-pic1
  2. If using random hexamers, incubate the reaction mixture at 25°C for 10 minutes, then proceed to step 3.
  3. Incubate the reaction mixture at temperatures between 42°C to 55ºC for 30-60 minutes.
  4. Inactivate the reaction by incubating at 65°C for 20 minutes.
  5. Proceed to PCR amplification step.

igScript™ First Strand cDNA Synthesis Kit

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