igScript™ RT-PCR Kit combines two powerful mixtures: i). 5x igScript™ master mix and ii) Hot start Taq 2x master mix with standard buffer for 2-steps RT-PCR. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.
The first strand cDNA synthesis is achieved by using 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer
The amplification step features a high quality Hot start Taq DNA Polymerase which offers higher fidelity and better amplification in a master mix format. RT-PCR product can be generated up to 6 kb.