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ig SYBR Green qPCR 2X Master Mix

$65.00$250.00


    100 reactions - $65.00 (Cat.# ig3355 )

    500 reactions - $250.00 (Cat.# ig3356 )

Category: .

Description

Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

Applications

  • Gene expression data validation.
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • viral detection
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling

Benefits

  • Enhanced efficiency, specificity, and sensitivity
  • Compatible with all real-time PCR instruments
  • Superior gene expression results under various
    cycling conditions
  • Robust and active for cDNA synthesis at temperatures up to 55 °C.

Product Includes

  1.  ig™ SYBR Green qPCR 2x Master Mix

Storage Temperature
–20 °C

Note 

*The use of ROX dye is necessary for all Applied Biosystems instruments and is optional for the Stratagene Mx3000P™, Mx3005P™, and Mx4000™ cyclers. Bio-Rad, Qiagen, Eppendorf, Illumina and Roche instruments do not require ROX dye.

 

Additional information

reaction

100 reactions, 500 reactions

Applications

  • Gene expression data validation.
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • viral detection
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling

Benefits

  • Enhanced efficiency, specificity, and sensitivity
  • Compatible with all real-time PCR instruments
  • Superior gene expression results under various
    cycling conditions
  • Robust and active for cDNA synthesis at temperatures up to 55 °C.

Product Includes

  1.  ig™ SYBR Green qPCR 2x Master Mix

Storage Temperature
–20 °C

Note 

*The use of ROX dye is necessary for all Applied Biosystems instruments and is optional for the Stratagene Mx3000P™, Mx3005P™, and Mx4000™ cyclers. Bio-Rad, Qiagen, Eppendorf, Illumina and Roche instruments do not require ROX dye.

  1. Place kit components, primers and RNA samples on ice.
  2. Mix and then centrifuge briefly to collect contents at the bottom of the tube.
  3. Prepare a master mix for each reaction and control plus 10% extra to allow for pipetting error according to the following table:
  4. Mix the reaction mixture thoroughly.

sybr-green-pic-1

  1. Program the thermal cycler according to the manufacturer’s instructions.
  2. A typical PCR cycling program is outlined in the
    following table.
  3. Place the PCR tubes in the thermal cycler and start the cycling program.

sybr-green-pic-2

  1. Analyze the data according to manufacturer
    protocol.

** For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines

ig™ SYBR Green qPCR 2X Master Mix

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