ig SYBR Green qPCR 2X Master Mix


    100 reactions - $65.00 (Cat.# 3355. )

    500 reactions - $229.00 (Cat.# 3356. )

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Intact Genomics SYBR® Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.


  • Gene expression data validation.
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • viral detection
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling


  • Enhanced efficiency, specificity, and sensitivity
  • Compatible with all real-time PCR instruments
  • Superior gene expression results under various
    cycling conditions
  • Robust and active for cDNA synthesis at temperatures up to 55 °C.

Product Includes

  1.  ig™ SYBR Green qPCR 2x Master Mix

Storage Temperature
–20 °C


*The use of ROX dye is necessary for all Applied Biosystems instruments and is optional for the Stratagene Mx3000P™, Mx3005P™, and Mx4000™ cyclers. Bio-Rad, Qiagen, Eppendorf, Illumina and Roche instruments do not require ROX dye.3355 3356

Additional information


100 reactions, 500 reactions

1. Place kit components, primers and RNA samples on ice.
2. Mix and then centrifuge briefly to collect contents at the bottom of the tube.
3. Prepare a master mix for each reaction and control plus 10% extra to allow for pipetting error according to the following table:

SYBR Green qPCR setup








4. Mix the reaction mixture thoroughly.
5. Program the thermal cycler according to the manu-facturer’s instructions.
6. A typical PCR cycling program is outlined in the following table.

7. Place the PCR tubes in the thermal cycler and start the cycling program.
8. Analyze the data according to manufacturer protocol.
** For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines.


Manual - ig™ SYBR Green qPCR 2X Master Mix

MSDS - ig SYBR Green qPCR 2x Master Mix

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