Description

Intact Genomics SYBR® Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

Applications

  • Gene expression data validation.
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • viral detection
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling

Benefits

  • Enhanced efficiency, specificity, and sensitivity
  • Compatible with all real-time PCR instruments
  • Superior gene expression results under various
    cycling conditions
  • Robust and active for cDNA synthesis at temperatures up to 55 °C.

Product Includes

  1.  ig™ SYBR Green qPCR 2x Master Mix

Storage Temperature
–20 °C

Note 

*The use of ROX dye is necessary for all Applied Biosystems instruments and is optional for the Stratagene Mx3000P™, Mx3005P™, and Mx4000™ cyclers. Bio-Rad, Qiagen, Eppendorf, Illumina and Roche instruments do not require ROX dye.3355 3356