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Pfu DNA Polymerase

$115.00$540.00


    200 Units (40 μl) - $115.00 (Cat.# 3312 )

    1,000 Units (200 μl) - $490.00 (Cat.# 3314 )

    200 Units (40 μl) include dNTP - $129.00 (Cat.# 3312d )

    1,000 Units (200 μl) include dNTP - $540.00 (Cat.# 3314d )

Category: .

Description

Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures (1). This product is supplied with the unique Intact Genomics 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM, and 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).

pfu-pic1

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Pfu Polymerase Comparison Data

We repeatedly compare our Pfu Polymerase side-by-side with a leading competitor for PCR assay. Our enzyme is better than the competitor.  A typical gel picture is shown below:

Picture

Applications

  • Routine PCR
  • Primer extension
  • Colony PCR
  • Genotyping
  • Efficient for amplifying high GC content template DNA with Magic Enhancer

 Product Includes

  1. Pfu DNA Polymerase
  2. 10x PCR Buffer with Mg2+
  3. 5x Magic Enhancer
  4. 10 mM dNTP (Cat. # 3312d, 3314d only)

Storage Temperature
–20 °C

Storage Buffer

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 ºC. 

10X PCR Buffer with Mg2+

100 mM Tris-HCl pH 9.0, 15 mM MgCl2, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.

 

Additional information

Units

200 Units (40 μl), 1,000 Units (200 μl), 200 Units (40 μl) include dNTP, 1,000 Units (200 μl) include dNTP

  1. Thaw 10x PCR Buffer, dNTP mix, primer solutions, 5X Magic Enhancer (if required) and mix thoroughly before use.
  2. Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.
  3. PictureMix the reaction mixture thoroughly.
  4. Add template DNA to the individual PCR tubes containing the reaction mixture.
  5. Program the thermal cycler according to the manufacturer’s instructions. A typical PCR cycling program is outlined in the following table.

Picture

  1. Place the PCR tubes in the thermal cycler and start the cycling program.

 Reference

  1. Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.

Pfu DNA Polymerase Manual

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