Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C
RNase Inhibitor, Murine
RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. RNase Inhibitor from Murine is resistance to oxidation compared to the human/porcine RNase inhibitors as it lacks the pair of cysteines found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).
E. coli strain expressing recombinant RNase Inhibitor gene from mouse.
- RNase Inhibitor, Murine
- cDNA synthesis
- In vitro transcription/translation
- RNA labeling
One unit is defined as the amount of RNase Inhibitor is required to inhibit the activity of 5 ng of RNase A by 50%.
70ºC for 20 minutes
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
pH 7.5 @ 25ºC
Quality Control assays
SDS-PAGE purity, protein concentration, endo-deoxyribonuclease assay, performance evaluation with RT-qPCR
RNase Inhibitor (RI) Data Comparison
RT-qPCR was run using Intact Genomics (IG) 1-step 2x master mix (MM) and compared IG RI with a leading brand’s “N” RI.
4,000 units, 20,000 units