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RNase Inhibitor, Murine

$75.00$295.00

Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C


    4,000 units - $75.00 (Cat.# 3711 )

    20,000 units - $295.00 (Cat.# 3714 )

Description

RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. RNase Inhibitor from Murine is resistance to oxidation compared to the human/porcine RNase inhibitors as it lacks the pair of cysteines found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.

Protein Purity
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).
RNase Inhibitor Purity

 

 

 

 

 

 

 

Product Source
E. coli strain expressing recombinant RNase Inhibitor gene from mouse.

Product Includes

  • RNase Inhibitor, Murine

Applications

  • cDNA synthesis
  • RT-PCR/RT-qPCR
  • In vitro transcription/translation
  • RNA labeling

Unit Definition
One unit is defined as the amount of RNase Inhibitor is required to inhibit the activity of 5 ng of RNase A by 50%.

Storage Temperature
20ºC

Heat Inactivation
70ºC for 20 minutes

Storage Buffer
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC

Storage Temperature
20ºC

Quality Control assays
SDS-PAGE purity, protein concentration, endo-deoxyribonuclease assay, performance evaluation with RT-qPCR

RNase Inhibitor (RI) Data Comparison
RT-qPCR was run using Intact Genomics (IG) 1-step 2x master mix (MM) and compared IG RI with a leading brand’s “N” RI.

Rase Inhibitor Compare

4714 4711

Additional information

units

4,000 units, 20,000 units

Manual - RNase Inhibitor

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