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T4 DNA Helicase (gp41)

$350.00$1,025.00


    100 µg (0.1 μg/μl) - $350.00 (Cat.# 3552 )

    500 µg (0.1 μg/μl) - $1025.00 (Cat.# 3555 )

Category: .

Description

T4 DNA helicase (gp41) is a hexameric, DnaB-like helicase that unwinds DNA with 5′ to 3′ polarity (1-3). Helicase activity has been shown to be stimulated upon association with gp61 or in coupled assays with the T4 DNA polymerase gp43 (4, 5). T4 replication helicase (gp41) and polymerase (gp43) can carry out ATP-dependent strand displacement DNA synthesis at physiological rates with high processivity in the absence of other T4 DNA replication proteins.

 Protein Purity

The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).

Product Source

  1. coli BL21 (DE3) strain expressing T4 gp41 gene.

Product Includes

  • gp41 protein
  • 10X gp41 Reaction Buffer

 

1x gp41 reaction buffer composition

20 mM Tris-acetate
100 mM Potassium acetate
10 mM Magnesium acetate
1 mM DTT
pH 7.8 @ 25ºC

Storage Buffer

50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC

Storage Temperature

20ºC

Quality Control assays

gp41 is free from detectable nuclease activities.

References

  1. Dong F, Gogol EP, von Hippel PH. J Biol Chem. 1995;270:7462–7473
  2. Patel SS, Picha KM. Annu Rev Biochem. 2000;69: 651–697
  3. Venkatesan M, Silver LL, Nossal NG. J Biol Chem. 1982;257: 12426–12434
  4. Schrock RD, Alberts B. J Biol Chem. 1996;271:16678–16682.
  5.  Richardson RW, Nossal NG. J Biol Chem. 1989; 264:4725–4731

Additional information

µg

100 µg (0.1 μg/μl), 500 µg (0.1 μg/μl)

T4 DNA Helicase (gp41)