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T4 gp46 Protein

$185.00$895.00


    100µg (2µg/µl) - $185.00 (Cat.# 3522 )

    500µg (2µg/µl) - $895.00 (Cat.# 3525 )

Category: .

Description

Spontaneous double-strand break (DSB) is one of the most deleterious forms of DNA damage, and their improper repair can lead to cellular dysfunction. The Mre11 (a nuclease) and Rad50 (an ATPase) form a well-conserved MR complex that is involved in the initial processing of DSB (1). The T4 gp47 protein and T4 gp46 protein complex is required for homologous recombination and DSB repair (2). The physiological function of the MR complex is a DNA nuclease, which is carried out by the Mre11 subunit. T4 Rad50 (gp46) is always required for the activity of T4 Mre11(gp47) but ATP is only activating when repetitive exonuclease activity is assayed, suggesting that ATP hydrolysis is involved in the translocation of the MR complex along the DNA substrate (3).

 Protein Purity

The physical purity of T4 gp46 Protein is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).

Product Source

  1. coli BL21 (DE3) strain expressing T4 gp46 gene.

Product Includes

  • T4 gp46 protein
  • 10X gp46 Reaction Buffer

1x gp46 reaction buffer composition

50 mM Tris-HCl
50 mM KCl
5 mM MgCl2
0.3 mM MnCl2
0.1 mg/ml BSA
pH 7.6 @ 25ºC

Storage Buffer

50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC

Storage Temperature

20ºC

Quality Control assays

Gp46 is free from detectable nuclease activities.

References

  1. Buis J., Wu Y., Deng Y., Leddon J., Westfield G., Eckersdorff M., Sekiguchi J. M., Chang S., Ferguson D. O. (2008) Cell 135, 85–96
  2. Kreuzer K. N., Yap W. Y., Menkens A. E., Engman H. W. (1988) J. Biol. Chem. 263, 11366–11373
  3. Herdendorf T. J., Albrecht D. W., Benkovic S. J., Nelson S. W. (2011) J. Biol. Chem. 286, 2382–2392

Additional information

µg

100µg (2µg/µl), 500µg (2µg/µl)

T4 gp46 Protein