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T4 UvsX DNA Recombinase

$295.00$675.00


    100µg (2μg/μl) - $295.00 (Cat.# 3562 )

    500µg (2μg/μl) - $675.00 (Cat.# 3565 )

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Description

Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. UvsX and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange (1-3).

 Protein Purity

The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).

 

 

Additional information

µg

100µg (2μg/μl), 500µg (2μg/μl)

Product Source

  1. coli BL21 (DE3) strain expressing T4 UvsX gene.

Product Includes

  • UvsX Recombinase
  • 10X UvsX Recombinase Reaction Buffer

 

1x UvsX Recombinase reaction buffer composition

20 mM Tris-acetate pH 7.8

100 mM Potassium acetate

10 mM Magnesium acetate

1 mM DTT

 

Storage Buffer

50 mM Tris-HCl

50 mM KCl

1 mM DTT

0.1 mM EDTA

50% Glycerol

pH 7.5 @ 25ºC

 

Storage Temperature

20ºC

Quality Control assays

UvsX recombinase is free from detectable nuclease activities.

References

  1. Cromie GA, Connelly JC, Leach DR (2001) Recombination at double-strand breaks and DNA ends: conserved mechanisms from phage to humans. Mol Cell 8: 1163–1174
  2. Michel B, Grompone G, Flores MJ, Bidnenko V (2004) Multiple pathways process stalled replication forks. Proc Natl Acad Sci U S A 101: 12783–12788
  3. Liu J, Ehmsen KT, Heyer WD, Morrical SW (2011) Presynaptic filament dynamics in homologous recombination and DNA repair. Crit Rev Biochem Mol Biol 46: 240–270

T4 UvsX DNA Recombinase