Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange (1-3).
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).
- Ecoli BL21 (DE3) strain expressing T4 UvsX gene.
- UvsX Recombinase
- 10X UvsX Recombinase Reaction Buffer
1x UvsX Recombinase reaction buffer composition
20 mM Tris-acetate pH 7.8
100 mM Potassium acetate
10 mM Magnesium acetate
1 mM DTT
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
pH 7.5 @ 25ºC
Quality Control assays
UvsX recombinase is free from detectable nuclease activities.
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- Michel B, Grompone G, Flores MJ, Bidnenko V (2004) Multiple pathways process stalled replication forks. Proc Natl Acad Sci U S A 101: 12783–12788
- Liu J, Ehmsen KT, Heyer WD, Morrical SW (2011) Presynaptic filament dynamics in homologous recombination and DNA repair. Crit Rev Biochem Mol Biol 46: 240–270