T7 RNA Polymerase is useful for sgRNA and mRNA synthesis, RNA synthesis from DNA templates and applications in mRNA therapeutics
T7 RNA Polymerase
$149.00
Description
Intact Genomics Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the bacteriophage T7 promoter and terminator sequences. T7 RNA Polymerase catalyzes in vitro RNA synthesis in a 5’→3′ direction from a cloned DNA sequence under the T7 promoters. The enzyme can incorporate labeled or unlabeled nucleotide triphosphates into an RNA transcript.
Protein Purity
The physical purity of this enzyme is ≥95% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
Applications
- Radiolabeled RNA probe preparation
- RNA synthesis from DNA template
- sgRNA and mRNA synthesis
- Applications in mRNA therapeutics
Product Includes
1) T7 RNA Polymerase
2) 10X T7 RNA Polymerase reaction buffer
Storage Temperature
-20°C
10x T7 RNA Polymerase reaction buffer
400 mM Tris-HCl
60 mM MgCl2
10 mM DTT
20 mM spermidine
pH 7.9 @ 25°C
Storage Buffer
50 mM Tris-HCl
100 mM NaCl
20 mM β-ME
1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100
pH 7.9 @ 25°C
Quality Control assays
T7 RNA polymerase is free from detectable nuclease activities.3723
Additional information
| Units | 25,000 Units |
|---|
Protocol
- Add the following components to make the reaction mixture:
- Incubate at 37°C for 60 minutes. For shorter transcripts (< 300 nt), incubate at 37°C for at least 2 hours.
