Call Us Today! 1-855-835-7172|sales@intactgenomics.com

Taq DNA Ligase

$50.00$195.00


    2,000 units (50 µl) - $50.00 (Cat.# 3218 )

    10,000 units (250 µl) - $195.00 (Cat.# 3219 )

Category: .

Description

Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. Taq DNA Ligase is active at elevated temperatures (45°C-70°C) (1, 2).

The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).

Desc

 

 

 

 

 

 

 

Additional information

Units

2,000 units (50 µl), 10,000 units (250 µl)

Product Source
E. coli strain expressing the cloned Taq DNA ligase gene from Thermus aqauticus HB8

Applications

  • Allele-specific gene detection by using Ligase Detection Reaction (LDR) and Ligase Chain Reaction (LCR) (1).
  • Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification(3).

Product Includes
1)   Taq DNA Ligase
2)   10X Taq DNA Ligase Buffer with NAD+ 

Storage Temperature
–20 °C

Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA,
50% Glycerol, pH 7.5 @ 25 ºC

10X Taq DNA Ligase reaction buffer with NAD+

500 mM Tris-HCl, 100 mM MgCl2, 100 mM DTT, 10 mM NAD+, pH 7.5 @ 25ºC

Unit Definition

One unit is defined as the amount of Taq DNA Ligase required to join 50% of 1 μg of the 12-base cohesive ends of Lambda DNA cut with Sma I and Sal I in 50 μl reaction in 15 min incubation at 45 °C.

Quality Control

Taq DNA Ligase is free from detectable RNase or contaminating DNA endonuclease activities.

  1. Set-up the reaction as follows:

Protocol

2. Incubate at 50 °C for 15-30 minutes.

Reference

  1. Barany, F. (1991). Proc. Natl. Acad. Sci. USA. 88, 189-193.
  2. Takahashi, M. et al. (1984). J. Biol. Chem. 259, 10041-10047.
  3. Michael, S.F. (1994). Biotechniques. 16, 411-412.

Taq DNA Ligase

You may also like…