Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.
Intact Genomics Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1.
- Primer extension
- Colony PCR
- Taq DNA Polymerase 2x Premix with Dye
- 5x Magic Enhancer
1x Premix Composition
10 mM Tris-HCl pH 9.0 , 50 mM KCl, 1.5 mM MgCl2, 0.2 mM dNTP, 5% Glycerol, 0.08% Igepal CA 630, 0.05% TWEEN-20, 25 Units/ml Taq DNA Polymerase
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.3248 3249