FastAmp® Viral and Cell Solution for Covid-19 Testing

$300.00$5,100.00

FastAmp® Viral and Cell Solution for Covid-19 streamlines viral specimen collection and viral RNA preparation processes including sample collection, transport, maintenance, viral/cell lysis, and viral RNA stabilization.


    100 reactions (50mL) - $300.00 (Cat.# 4631 )

    2,000 reactions (1,000 mL) - $5100.00 (Cat.# 4633 )

Category: .

Description

For research use only.  Not to be used for diagnostic purposes.
Covid-19 Viral SolutionIntact Genomics proprietary FastAmp® Viral/Cell Solution can be utilized for saliva/swab samples resulting in viral/cell lysis, and significantly simplifying this process. This solution streamlines viral specimen collection and viral RNA preparation processes including sample collection, transport, maintenance, viral/cell lysis, and viral RNA stabilization. After using the solution, the lysed viral RNA may be directly used in RT-qPCR, RT-isothermal DNA amplification, or any direct diagnosis kits for Covid-19 testing, without the need of an RNA extraction step.
FastAmp® Viral/Cell Solution for Covid-19 Testing can be used to detect SARS-CoV-2 in any type of respiratory specimens such as swabs, saliva, sputum, nasopharyngeal, oropharyngeal aspirates, washes or/and tracheal aspirates.
This solution allows for efficient viral sample collection, processing, and SARS-CoV-2 viral RNA preparation in a single tube. It makes Covid-19 testing simpler, faster, safer and more affordable.

Product Includes:
       FastAmp® Viral/Cell Solution

Benefits:

  • No RNA extraction is needed
  • Safe for sample transport and maintenance
  • Speeds up the Covid-19 testing process
  • Compatible with all type of viral specimens
  • Compatible with different detection technologies
  • Increased efficiency, specificity, and sensitivity
  • Low toxicity to humans/environment

Storage Temperature: Room temperature

Quality Control:
Functionally tested in RT-qPCR based on CDC recommended probe for activity, processivity, efficiency and sensitivity.

Safety Statement:
This kit is intended for Research Use Only (RUO). It has been repeatedly tested by Intact Genomics and other institutes, but not been tested and validated by any public health agency.

Innovation:
Part of what makes Intact Genomics FastAmp® Viral/Cell Solution for Covid-19 Testing an innovative product is the fact that it contains very low concentration of less toxic chemicals different from others. The CDC currently recommends viral lysis buffers and/or RNA extraction solutions from Qiagen, Roche, and other companies. These products are not only expensive, but also contain a high concentration of chemicals known to be toxic to humans and the environment. These harmful components include:

  • 30%~70% guanidinium thiocyanate/guanidine hydrochloride (several QIAmp RNA Kits, NUCLISENS® easyMAG® Lysis Buffer),
  • thiocyanic acid/guanidine (Roche MagNA Pure 96 External Lysis Buffer) [1-4],
  • phenol-containing extraction agents such as Trizol [5].

The Trizol/virus mixture can be stored at -70oC for an extended amount of time, however, viral RNA is not stable in these reagents during this period, particularly at room temperature. Therefore, in order to remove all toxic chemicals, an expensive RNA preparation kit is required [1-4].

Intact Genomics FastAmp® Viral/Cell Solution makes Covid-19 testing simpler, faster, safer, more affordable, and environment-friendly.

References
1.https://www.fda.gov/media/134922/download.

2.https://sds.qiagen.com/ehswww/QIAGENwww/result/result_htmllist.jsp?P_LANGU=E&P_SYS=4&P_SSN=42571&C001=MSDS&C700=KIT&C007=PROD&C100=QIA&C002=US&C013=61904.

3.https://lifescience.roche.com/en_us/products/magna-pure-96-external-lysis-buffer.html#documents.

4.https://biomerieuxdirect.com/clinical/Molecular-Biology/Molecular-Biology/Nuclisens/NucliSens-Reagents-Lysis-Extraction/Nuclisens-Reagent-and-Consumable/NUCLISENS-LYSIS-BUFFER-4X1L/p/280134.

5.Blow JA, Dohm DJ, Negley DL, Mores CN (2004) Virus inactivation by nucleic acid extraction reagents. J Virol Methods 119:195-198.4631 4633

Additional information

reaction

100 reactions (50mL), 2,000 reactions (1,000 mL)

Protocol:

  1. Collect a sample from 1). saliva (~300 µl of saliva); or 2). nose by a swab
  2. Resuspend 50 µl of saliva or the swab containing the suspected COVID-19 virus in FastAmp® Viral/Cell Solution (500 µl) to lysis the viral/cell and obtain the lysed viral/cell mixture.
  3. Incubate this mixture at room temperature for 5 minutes. The lysed viral/cell mixture can also be stored or transported long-term (months) at refrigerated temperature (-20°C or -80°C).
  4. Add 1~5 µl of the lysed viral/cell mixture directly to one-step RT-qPCR kit (e.g. Intact Genomics, Cat# 4223) for SARS-CoV-2 detection.

 

Manual FastAmp® Viral and Cell Solution for Covid-19 Testing

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