RPA Optimization Enzymes main imageIntact Genomics offers a variety of RPA optimization products.  RPA is an excellent candidate for developing rapid point-of-care molecular testing tools as well as agricultural applications, clonal amplification in next-generation sequencing workflows, and more.

Recombinase polymerase amplification (RPA) amplifies DNA at a constant temperature (37-42 °C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filament. This complex is able to bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau polymerase extends the primers, creating a new complete copy of the template, and amplification can continue like Polymerase Chain Reaction (PCR).

Recombinase Polymerase Amplification details

RPA vs PCR table

Intact Genomics offers a variety of RPA optimization enzymes. We are continuously discovering and developing novel classes of RPA enzymes in order to speed up the point-of-care (POC) diagnostic process.

Applications and Benefits:

• Highly selective and sensitive isothermal amplification technique.
• Alternative to PCR. No Thermocycler needed.
• Speed and sensitivity. Excellent for Rapid Molecular and Agricultural tests. This isothermal reaction employing T4 UvsX DNA Recombinase does not require pretreatment of DNA, nor does it require any strand denaturation, making it suitable for field tests or performing tests with minimal equipment and supplies.
• Successfully implementation with different detection strategies, from end-point lateral flow strips to real-time fluorescent detection.
• All enzymes thoroughly tested for activity and purity.