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igScript™ One Step RT-qPCR Kit

$195.00$695.00


    100 reactions (20 µl rxn vol) - $195.00 (Cat.# 4214 )

    500 reactions (20 µl rxn vol) - $695.00 (Cat.# 4218 )

Description

igScript™ one step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55ºC.

ig™ SYBR Green qPCR 2X master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The ig SYBR® Green qPCR 2X master mix with integrated chemically-modified hot start Taq polymerase, SYBR® Green I fluorescent dye, MgCl2, dNTPs and stabilizers. ROX reference dye is not included in the 2X master mix. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

Applications

  • Gene expression data validation.
  • Multiplexing
  • Mutation detection
  • Pathogen and viral detection
  • Genetically modified organisms (GMO) characterization and Genetic profiling

 Benefits

  • Enhanced efficiency, specificity, and sensitivity
  • Compatible with all real-time PCR instruments
  • Superior gene expression results under various cycling conditions
  • Robust and active for cDNA synthesis at temperatures up to 55°C.

Product Includes

  1. ig Script™ Reverse Transcriptase
  2. ig SYBR™ Green qPCR 2x Master Mix

 Storage Temperature
–20 °C

Additional information

reaction

100 reactions (20 µl rxn vol), 500 reactions (20 µl rxn vol)

Protocol

  1. Place kit components and RNA samples on ice.
  2. Mix and then centrifuge briefly to collect contents at the bottom of the tube.
  3. Prepare a master mix for each reaction and control requiring Reverse Transcriptase enzyme plus 10% extra to allow for pipetting error according to the following table:

igscript-one-step-rt-qpcr-kit-pic1

  1. Mix the reaction mixture thoroughly.
  2. Program the thermal cycler according to the manufacturer’s instructions.
  3. A typical PCR cycling program is outlined in the following table.
  4. Place the PCR tubes in the thermal cycler and start the cycling program.

pfu-2x-master-mix-pic3

  1. Analyze the data according to manufacturer protocol.

* For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines.

igScript™ One Step RT-qPCR Kit Manual

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